10 Safety Measures Every Microbiology Professional Should Know

10 Safety Measures Every Microbiology Professional Should Know

In the initial material of your laboratory manual, you can find some of these rules and procedures. Please overcome this by asking your teacher or assistant to teach (TA) for clarification in the event that any safety rules or procedures mentioned here tend to conflict with those stated in your laboratory manual.

Microbial protection in a laboratory is critical in infection prevention because it manipulates and uses virulent and/or potentially pathogenic microorganisms in the microbiology laboratory.

10 Safety Measures Every Microbiology Professional Should Know

Microbiology is a science that explores microscopic biology. While individual cells of these species can be examined directly by microscopes and their structures and behaviours are studied, the preferred method is to analyse other characteristics such as metabolism or genetics. Both cells in the population must be practically similar in certain forms of experiments: pure cultures are called such populations.

Besides microbial materials, certain potentially harmful chemicals are used in this laboratory. Many operations include glassware, flames open and sharp objects which, if misuses, may cause trauma/damage.

1. Treat every micro-organism as a Potential Pathogen

While the majority of microorganisms are human not pathogenic or disease-causing, certain microorganisms that are not usually pathogenic can be pathogens under unusual conditions.

Treat all micro-organisms — especially unknown cultures — as pathogenic. A student with an impaired or recent extended disease immune system should speak to the teacher before working in the microbiology laboratory.

2. Clean work area before and after use

To wipe benches and places, both before and after work with cultures, use a disinfectant, such as a ten percent bleach or 70 percent ethanol solution.

Know also the potential hazards in the disinfectant, since 70% of ethanol will fire at an open fire or high heat. Bleach will ruin your clothes if it’s spilt. If splashed into the eyes, either alcohol or bleach can be harmful. Students should be conscious of the location of the nearest washing station and sink.

3. Sterilization

Equipment and supplies for sterilisation. Autoclaving can sterilise all materials, media, tubes, plates, loops, needles, pipettes and other products of cultivation of microorganisms. Use sterilised goods in the company, otherwise. Know how all equipment and materials needed for laboratory operations and safe use.

4. Wash your hands.

Using a disinfectant cleaner before and after work with microorganisms to wash your hands. Non-disinfectant soap can kill bacteria in the surface which can be used unless there is a disinfectant soap. You should wear gloves as added protection.

5. Never mouth pipette

For aspiration and dispensing of liquid crops, use pipette or pipette machines.

6. Do not eat in Lab

Do not feed, drink or store food in the lab in areas that store micro-organisms. Never eat or beverage with microorganisms in the laboratory. Before and after laboratory operations, keep your fingers out of your mouth, and wash your face.

Cover with a bandage any wounds on your side. You should wear gloves as added protection.

7. Autoclave all waste material or clean it

All the products to be discarded in the course should be put into an autoclave bag with biohazard and autoclaved at 20 kilos of pressure for 30 to 40 minutes at 121 ° C, e.g. culture tube, crop plates, swabs, dentures, wipes, disposable transfer needles, and gloves.

If no autoclave is available and no bacteria are used, the materials should be coated with a 10% bleach solution and can be soaked throughout at least 1 to 2 hours.

8. Accidents and Injuries

Notify the teacher immediately about any accident or injury (spill, crack, etc.), regardless of how insignificant it might sound.

When a culture is spilt or lost, put a paper towel over the spill and pour the disinfectant on the towel after notifying the trainer. Wait 15 minutes before purifying the spill with fresh towels of paper. Rest assembled towels and wash your hands carefully in your wastebasket.

9. Disposal

  1. Discard all cultures, Petri-plates, and used glassware in the container labelled contaminated or biohazard. This container will later be autoclaved.
  2. NEVER place contaminated pipettes on the benchtop.
  3. NEVER place contaminated inoculating loops on the benchtop.
  4. NEVER discard contaminated cultures, Petri dishes, glassware, pipettes, tubes, or slides in the trash can.
  5. NEVER discard contaminated liquids or liquid cultures in the sink.

10. Placement of Items

During and at the end of each lab period, used pipets should be discarded into the designated trays. Other used glassware should normally be placed into discard trays
located in the back of the lab.

Plasticware such as Petri plates and pipette tips should be
discarded in the marked wastebasket at the back of the lab. The used paper should be discarded into wastebaskets at the end of the lab period. Broken glassware is discarded into the box marked “Broken Glassware”, not into wastebaskets.

10 Safety Measures Every Microbiology Professional Should Know

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