How to extract DNA from almost all living things
First, anything containing DNA has to be identified. DNA, as it is life’s blueprint, includes everything that lives DNA.
We like to use green split peas for this experiment. But several other DNA sources are also available, for example:
- Liver of chicken
- Fresh fruit
Other DNA origins are not to be used, for example:
- Fido the puppy, your pet’s skin
- The big toe of your little sister
- You found bugs in the courtyard
Phase 1: Insanity Blender!
Bring a mixer in:
Split peas 1/2 cup (100ml)
1/8 salt (fewer than 1 ml) teaspoon table
1 cup (200ml) of ice water
Fifteen seconds mix on strong.
The processor removes the pea cells, and you get a really small pea cell broth.
Phase 2: Peas Soapy
Pour the good pea cell soup in another pan (such as a cup of measurement), using a strainer.
Add 2 teaspoons (approximately 30 ml) of liquid detergent and whirl to blend.
Rest 5-10 minutes in the mixture.
Pour the mixture into test tubes or other small containers of water, each roughly 1/3 complete.
Phase 3: Control of the Enzyme
Fill each tubing with a pinch of enzymes and mix gently. Pay heed! You will cross the Genome, making it easier to see if you work too fast.
Using enzyme beef tenderizer. Consider wiping pineapple juice or eye touch if you can’t find a tenderizer.
Phase 4: Alcohol Separation
Slowly turn the test tube into the container and place it in a film on top of the pea mixture to produce rubbing alcohol of 70-95% isopropyl or ethyl alcohol. Sprinkle in the tube until the pea mixture is about the same amount of alcohol.
Alcohol is less compact than water and is thus flowing around. Seek clumps of white material, where the layers of water and alcohol cross.
What’s the stringy thing?
DNA’s a long bundle of lines. The salt you have applied in step one helps to keep things together. So it’s clumps of DNA molecules that you see!
DNA is normally dissolved in water, but it is unclear if salty DNA comes into contact with alcohol. It is referred to as precipitation. The brute pressure of the DNA clustering as it precipitates draws more strands into the alcohol.
The DNA may be extracted using a wooden stick or a tube. You should transfer it to a little bottle filled with alcohol if you want to preserve the DNA.
DNA extraction has just been done!
You will now be able to try again because you have successfully removed DNA from one source. Submit these or your own ideas:
Any other DNA sources experiment. What is the most DNA source for you? Why are you able to compare them?
Experiment for various detergents and soaps. Will soap powder and detergents work as well? What about body wash or shampoo?
Experiment with actions that exit or alter. You need every step, we told you, but is that true? Find out yourself. Find out for yourself. Seek to take a step away or to change the sum of any product you use.
Would you want to do more research on DNA extraction? Check different detergents and cleaners. Will soap powder and detergents work as well?
Find out and let us know what worked and why? How to extract DNA from almost all living things