genome-assembly Questions – Bioinformatics India

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Genome assembly with Flye for long reads: what coverage depth is needed for a good assembly?

I’m assembling a bacterial genome (~4.5 Mb) using Oxford Nanopore reads with Flye. I have about 15x coverage right now. The assembly is fragmented (150+…

bacteria flye genome-assembly long-reads nanopore asked 4 weeks ago by

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How to estimate genome size from sequencing reads using k-mer analysis with Jellyfish and GenomeScope

I have Illumina WGS reads for a non-model organism and want to estimate the genome size before assembly. I’ve heard about k-mer frequency analysis. How…

genome-assembly genome-size genomescope jellyfish kmer asked 1 month ago by

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