rna-seq Questions – Bioinformatics India

41

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What is the best way to normalize RNA-seq count data before differential expression analysis?

I’m doing differential expression analysis with DESeq2 in R. I have raw count data from featureCounts. Should I normalize the counts before passing them to…

deseq2 differential-expression normalization r-programming rna-seq asked 5 days ago by

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52

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What is the difference between TPM, FPKM, and RPKM in RNA-seq gene expression normalization?

I keep seeing TPM, FPKM, and RPKM used in RNA-seq papers and tools. What is the mathematical difference between them? Which should I use for…

fpkm gene-expression normalization rna-seq tpm asked 3 weeks ago by

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41

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How to align RNA-seq reads using STAR aligner — genome indexing to sorted BAM

I need to align paired-end RNA-seq reads to the human GRCh38 reference genome using STAR. What are the steps to: (1) generate the genome index,…

read-alignment rna-alignment rna-seq splicing star asked 2 months ago by

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