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How to handle batch effects in scRNA-seq data using Seurat?

I’m integrating scRNA-seq datasets from 3 different batches (different labs, same tissue type). After merging in Seurat, the UMAP clusters by batch rather than by…

batch-correctionr-programmingscrna-seqseuratsingle-cell asked 3 weeks ago by Admin
56
1 answer ✓ 74 views

Complete single-cell RNA-seq analysis pipeline in Seurat from CellRanger output to cell type annotation

I have 10x Genomics scRNA-seq data processed through CellRanger. I now have the filtered_feature_bc_matrix output. What is the complete Seurat workflow from loading data to…

cell-type-annotationr-programmingscrna-seqseuratsingle-cell asked 4 weeks ago by Admin
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